> For the complete documentation index, see [llms.txt](https://cgps.gitbook.io/pathogenwatch/llms.txt). Markdown versions of documentation pages are available by appending `.md` to page URLs; this page is available as [Markdown](https://cgps.gitbook.io/pathogenwatch/technical-descriptions-of-analysis-tools/lineage-and-genotyping/ng-mast.md).

# NG-MAST

## About

NG-MAST (*Neisseria gonorrhoeae* multi-antigen sequence typing) is a typing scheme for *Neisseria gonorrhoeae* based on regions of the DNA sequences of the genes encoding the highly variable PorB and TbpB membrane proteins. As with MLST, each unique sequence of each gene fragment is assigned an allele number, and all observed unique combinations of the two alleles is assigned a NG-MAST number.

Pathogenwatch generates *in silico* assignments using the `ngmaster` script of[ Kwong et al](https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5320595/), which is available at <https://github.com/MDU-PHL/ngmaster>.

## How to cite

Kwong, J. C., Gonçalves da Silva, A., Dyet, K., Williamson, D. A., Stinear, T. P., Howden, B. P., & Seemann, T. (2016). *NGMASTER:in silico* multi-antigen sequence typing for *Neisseria gonorrhoeae*. *Microbial genomics*, *2*(8), e000076. <https://doi.org/10.1099/mgen.0.000076>